|T000070, PIM Tracer
Compound Image

T000070

PIM Tracer

  • Description

    Formula:

    C43H42BF5N8O3

    avg. MolWt:

    824.66 u

    PAINS elements:

    none detected

    SMILES:

    display
  • Properties

    Store at:

    -80 °C

    Absorption:

    450 nm

    Emission:

    590 nm

    clogP:

    7.34
  • References

    Contributing Lab:

    Promega Corp., Madison

  • Experimental

    Assay window 9.29
    \(Z'\) 0.86
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}^{\,\mathrm{app}}\):

    111 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    PIM447

    \(\mathrm{EC}_{50}\):

    10 nM
    displacement curve
    raw data

    Comment: PIM1-NLuc

    Assay window 4.35
    \(Z'\) 0.9
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}^{\,\mathrm{app}}\):

    1532 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    PIM447

    \(\mathrm{EC}_{50}\):

    22 nM
    displacement curve
    raw data

    Comment: PIM2-NLuc

    Assay window 3.25
    \(Z'\) 0.91
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}^{\,\mathrm{app}}\):

    150 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    PIM447

    \(\mathrm{EC}_{50}\):

    5 nM
    displacement curve
    raw data

    Comment: NLuc-PIM3