|T000063, TAOK Tracer
Compound Image

T000063

TAOK Tracer

  • Description

    Formula:

    C52H59BF5N11O4

    avg. MolWt:

    1007.92 u

    PAINS elements:

    none detected

    SMILES:

    display
  • Properties

    Store at:

    -80 °C

    Absorption:

    450 nm

    Emission:

    590 nm

    clogP:

    8.09
  • References

    Contributing Lab:

    Knapp Lab, University of Frankfurt

  • Experimental

    Assay window 3.3
    \(Z'\) 0.95
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}\):

    1136 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    Staurosporine

    \(\mathrm{IC}_{50}\):

    645 nM
    displacement curve
    raw data

    Comment: NLuc-TAOK2

    Assay window 4.1
    \(Z'\) 0.97
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}\):

    1777 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    Staurosporine

    \(\mathrm{IC}_{50}\):

    67 nM
    displacement curve
    raw data

    Comment: NLuc-TAOK3

    Assay window 3.6
    \(Z'\) 0.9
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}^{\,\mathrm{app}}\):

    > 10 µM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    Staurosporine

    \(\mathrm{EC}_{50}\):

    809 nM
    displacement curve
    raw data

    Comment: NLuc-TAOK3; Although the parameters allow a classification as robust assay, the classification was changed to expert because fluctuations in quality were observed