|T000061, K12 Tracer

Compound Image

T000061

K12 Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

Assay window 4.2

\(Z'\) 0.82

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

105 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dinaciclib

\(\mathrm{EC}_{50}\):

584 nM

raw data

Comment: CDK1-NLuc + CycB1

Reference: source

Assay window 3.7

\(Z'\) 0.81

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

157 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dinaciclib

\(\mathrm{EC}_{50}\):

177 nM

raw data

Comment: CDK1-NLuc + CycE1

Reference: source

Assay window 3.92

\(Z'\) 0.75

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

212 nM

raw data

Tracer displacement

i

Compound used for displacement:

SNS-032

\(\mathrm{EC}_{50}\):

989 nM

raw data

Comment: CDK10-NLuc + CycL2

Reference: source

Assay window 4.07

\(Z'\) 0.9

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

217 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

\(\mathrm{EC}_{50}\):

165 nM

raw data

Comment: CDK11A-NLuc + CycK

Reference: source

Assay window 4.66

\(Z'\) 0.88

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

137 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

\(\mathrm{EC}_{50}\):

278 nM

raw data

Comment: CDK11B-NLuc + CycL2

Reference: source

Assay window 5.22

\(Z'\) 0.64

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

92 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

\(\mathrm{EC}_{50}\):

306 nM

raw data

Comment: CDK14-NLuc + CycY

Reference: source

Assay window 3.9

\(Z'\) 0.71

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

40 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

\(\mathrm{EC}_{50}\):

185 nM

raw data

Comment: NLuc-CDK15 + CycY

Reference: source

Assay window 4.11

\(Z'\) 0.71

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

146 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

\(\mathrm{EC}_{50}\):

42 nM

raw data

Comment: CDK16-NLuc + CycY

Reference: source

Assay window 4.26

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

121 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

\(\mathrm{EC}_{50}\):

51 nM

raw data

Comment: CDK17-NLuc + CycY

Reference: source

Assay window 7.53

\(Z'\) 0.76

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

126 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

\(\mathrm{EC}_{50}\):

467 nM

raw data

Comment: CDK18-NLuc + CycY

Reference: source

Assay window 4.64

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

164 nM

raw data

Tracer displacement

i

Compound used for displacement:

AT7519

\(\mathrm{EC}_{50}\):

2 µM

raw data

Comment: CDK3-NLuc + CycE1

Reference: source

Assay window 3.75

\(Z'\) 0.9

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

127 nM

raw data

Tracer displacement

i

Compound used for displacement:

Palbociclib

\(\mathrm{EC}_{50}\):

33 nM

raw data

Comment: NLuc-CDK4 + CycD1

Reference: source

Assay window 5.16

\(Z'\) 0.77

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

88 nM

raw data

Tracer displacement

i

Compound used for displacement:

Abemaciclib

\(\mathrm{EC}_{50}\):

2 nM

raw data

Comment: NLuc-CDK4 + CycD3

Reference: source

Assay window 3.53

\(Z'\) 0.82

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

77 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dinaciclib

\(\mathrm{EC}_{50}\):

734 nM

raw data

Comment: NLuc-CDK7 + pGEM

Reference: source

Assay window 11.59

\(Z'\) 0.93

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

13 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dinaciclib

\(\mathrm{EC}_{50}\):

11 nM

raw data

Comment: NLuc-CDK9 + CycK

Reference: source