|T000040, K3 Tracer

Compound Image

T000040

K3 Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

Assay window 5.75

\(Z'\) 0.82

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

27 nM

raw data

Tracer displacement

i

Compound used for displacement:

Alpelisib

\(\mathrm{EC}_{50}\):

21 nM

raw data

Comment: NLuc-PIK3CA + PIK3R1

Reference: source

Assay window 3.11

\(Z'\) 0.65

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

16 nM

raw data

Tracer displacement

i

Compound used for displacement:

PI-103

\(\mathrm{EC}_{50}\):

87 nM

raw data

Comment: NLuc-PIK3CA(C420R) + PIK3R1

Reference: source

Assay window 2.92

\(Z'\) 0.54

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

24 nM

raw data

Tracer displacement

i

Compound used for displacement:

PI-103

\(\mathrm{EC}_{50}\):

76 nM

raw data

Comment: NLuc-PIK3CA(E542K) + PIK3R1

Reference: source

Assay window 3.2

\(Z'\) 0.65

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

18 nM

raw data

Tracer displacement

i

Compound used for displacement:

PI-103

\(\mathrm{EC}_{50}\):

69 nM

raw data

Comment: NLuc-PIK3CA(E545A) + PIK3R1

Reference: source

Assay window 4.04

\(Z'\) 0.63

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

22 nM

raw data

Tracer displacement

i

Compound used for displacement:

PI-103

\(\mathrm{EC}_{50}\):

63 nM

raw data

Comment: NLuc-PIK3CA(E545K) + PIK3R1

Reference: source

Assay window 4.39

\(Z'\) 0.82

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

38 nM

raw data

Tracer displacement

i

Compound used for displacement:

PI-103

\(\mathrm{EC}_{50}\):

57 nM

raw data

Comment: NLuc-PIK3CA(H1047L) + PIK3R1

Reference: source

Assay window 4.04

\(Z'\) 0.54

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

27 nM

raw data

Tracer displacement

i

Compound used for displacement:

PI-103

\(\mathrm{EC}_{50}\):

67 nM

raw data

Comment: NLuc-PIK3CA(H1047R) + PIK3R1

Reference: source

Assay window 5.14

\(Z'\) 0.78

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

33 nM

raw data

Tracer displacement

i

Compound used for displacement:

PI-103

\(\mathrm{EC}_{50}\):

59 nM

raw data

Comment: NLuc-PIK3CA(H1047Y) + PIK3R1

Reference: source

Assay window 4.15

\(Z'\) 0.71

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

51 nM

raw data

Tracer displacement

i

Compound used for displacement:

PI-103

\(\mathrm{EC}_{50}\):

47 nM

raw data

Comment: NLuc-PIK3CA(I800L) + PIK3R1

Reference: source

Assay window 3.5

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

30 nM

raw data

Tracer displacement

i

Compound used for displacement:

PI-103

\(\mathrm{EC}_{50}\):

44 nM

raw data

Comment: NLuc-PIK3CA(Q546K) + PIK3R1

Reference: source

Assay window 4.35

\(Z'\) 0.76

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

184 nM

raw data

Tracer displacement

i

Compound used for displacement:

PI-103

\(\mathrm{EC}_{50}\):

35 nM

raw data

Comment: NLuc-PIK3CB + PIK3R1

Reference: source

Assay window 3.4

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

77 nM

raw data

Tracer displacement

i

Compound used for displacement:

Apitolisib

\(\mathrm{EC}_{50}\):

65 nM

raw data

Comment: NLuc-PIK3CD + PIK3R1

Reference: source