|T000038, K8 Tracer

Compound Image

T000038

K8 Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

Assay window 2.84

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

140 nM

raw data

Tracer displacement

i

Compound used for displacement:

ABT-702

\(\mathrm{EC}_{50}\):

5 nM

raw data

Comment: ADK-NLuc

Reference: source

Assay window 2.23

\(Z'\) 0.87

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

21 nM

raw data

Tracer displacement

i

Compound used for displacement:

AST-487

\(\mathrm{EC}_{50}\):

13 nM

raw data

Comment: NLuc-CDK19 + CycC

Reference: source

Assay window 2.52

\(Z'\) 0.9

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

22 nM

raw data

Tracer displacement

i

Compound used for displacement:

AST-487

\(\mathrm{EC}_{50}\):

38 nM

raw data

Comment: NLuc-CDK8 + CycC

Reference: source

Assay window 6.73

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

43 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dinaciclib

\(\mathrm{EC}_{50}\):

12 nM

raw data

Comment: NLuc-CDK9 + CycK

Reference: source

Assay window 4.02

\(Z'\) 0.79

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

69 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dinaciclib

\(\mathrm{EC}_{50}\):

16 nM

raw data

Comment: NLuc-CDK9 + CycT1

Reference: source

Assay window 3.21

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

72 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

\(\mathrm{EC}_{50}\):

757 nM

raw data

Comment: NLuc-ICK

Assay window 5.14

\(Z'\) 0.8

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

124 nM

raw data

Tracer displacement

i

Compound used for displacement:

AZD-5438

\(\mathrm{EC}_{50}\):

1 µM

raw data

Comment: NLuc-CSNK1D

Reference: source

Assay window 2.54

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

154 nM

raw data

Tracer displacement

i

Compound used for displacement:

AZD-5438

\(\mathrm{EC}_{50}\):

4 µM

raw data

Comment: NLuc-CSNK1E

Reference: source

Assay window 3.38

\(Z'\) 0.64

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

94 nM

raw data

Tracer displacement

i

Compound used for displacement:

CHIR-99021

\(\mathrm{EC}_{50}\):

55 nM

raw data

Comment: NLuc-GSK3A

Reference: source

Assay window 7.43

\(Z'\) 0.77

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

94 nM

raw data

Tracer displacement

i

Compound used for displacement:

CHIR-99021

\(\mathrm{EC}_{50}\):

120 nM

raw data

Comment: NLuc-GSK3B

Reference: source

Assay window 2.55

\(Z'\) 0.71

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

78 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

\(\mathrm{EC}_{50}\):

419 nM

raw data

Comment: KIT(V559D,T670I)-NLuc

Reference: source

Assay window 2.63

\(Z'\) 0.75

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

55 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI-7210

\(\mathrm{EC}_{50}\):

89 nM

raw data

Comment: PIKFYVE-NLuc

Reference: source

Assay window 3.14

\(Z'\) 0.72

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

117 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI-7210

\(\mathrm{EC}_{50}\):

99 nM

raw data

Comment: PIP4K2C-NLuc

Reference: source

Assay window 2.5

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

54 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI-7210

\(\mathrm{EC}_{50}\):

3 µM

raw data

Comment: NLuc-PIP5K1B

Reference: source

Assay window 2.79

\(Z'\) 0.84

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

72 nM

raw data

Tracer displacement

i

Compound used for displacement:

APY-69

\(\mathrm{EC}_{50}\):

972 nM

raw data

Comment: NLuc-RIPK1

Reference: source