|T000024, NCP399 (SAHA-FITC)
Compound Image

T000024

NCP399 (SAHA-FITC)

  • Description

    Formula:

    C36H34N4O8S

    avg. MolWt:

    682.76 u

    PAINS elements:

    none detected

    SMILES:

    display
  • Properties

    Store at:

    -20 °C

    Absorption:

    475 nm

    Emission:

    520 nm

    clogP:

    6.24
  • References

    Synthesis:

    Publication

    Contributing Lab:

    Mazitschek Lab, Massachusetts General Hospital, Boston

    Vendor:

    Tocris
  • Experimental

    Assay window 42.1
    \(Z'\) 0.93
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}\):

    42 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    SAHA

    \(\mathrm{IC}_{50}\):

    186 nM
    displacement curve
    raw data

    Comment: HDAC1(1-482(end)-His-FLAG), TR-FRET donor: CoraFluor-1

    Reference: PMID

    Assay window 45.5
    \(Z'\) 0.92
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}\):

    133 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    SAHA

    \(\mathrm{IC}_{50}\):

    597 nM
    displacement curve
    raw data

    Comment: HDAC2(1-488(end)-His), TR-FRET donor: CoraFluor-1

    Reference: PMID

    Assay window 131.2
    \(Z'\) 0.97
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}\):

    49 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    SAHA

    \(\mathrm{IC}_{50}\):

    59 nM
    displacement curve
    raw data

    Comment: HDAC3(1-428(end) in complex with NCOR2(395-489), TR-FRET donor: CoraFluor-1

    Reference: PMID