|T000019, VHL Tracer
Compound Image

T000019

VHL Tracer

  • Properties

    Store at:

    -80 °C

    Absorption:

    450 nm

    Emission:

    590 nm
  • References

    Contributing Lab:

    Promega Corp., Madison

    Vendor:

    Promega
  • Experimental

    Assay window 2.3
    \(Z'\) 0.73
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}\):

    484 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    VH298

    \(\mathrm{IC}_{50}\):

    279 nM
    displacement curve
    raw data

    Assay window 2.67
    \(Z'\) 0.7
    CP agreement yes
    Tracer titration

    \(K_\mathrm{D}^{\,\mathrm{app}}\):

    1604 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    VH298

    \(\mathrm{EC}_{50}\):

    279 nM
    displacement curve
    raw data

    Comment: VHL-NLuc

    Reference: source