|T000017, K9 Tracer

Compound Image

T000017

K9 Tracer

Properties

Store at:

-80 °C

Absorption:

450 nm

Emission:

590 nm
References

Contributing Lab:

Promega Corp., Madison

Vendor:

Promega
Experimental

Assay window 3.07

\(Z'\) 0.94

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

255 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

51 nM

raw data

Comment: NLuc-CAMK1

Reference: source

Assay window 2.83

\(Z'\) 0.88

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

255 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

51 nM

raw data

Comment: NLuc-CAMK1D

Reference: source

Assay window 3.29

\(Z'\) 0.89

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

316 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

\(\mathrm{EC}_{50}\):

832 nM

raw data

Comment: NLuc-CAMK1G

Reference: source

Assay window 3.77

\(Z'\) 0.82

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

460 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dinaciclib

\(\mathrm{EC}_{50}\):

137 nM

raw data

Comment: CDK1-NLuc + CycB1

Reference: source

Assay window 3.89

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

433 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dinaciclib

\(\mathrm{EC}_{50}\):

26 nM

raw data

Comment: CDK1-NLuc + CycE1

Reference: source

Assay window 2.68

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

258 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

44 nM

raw data

Comment: NLuc-CHEK1

Reference: source

Assay window 4.63

\(Z'\) 0.96

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

235 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

42 nM

raw data

Comment: CLK2-NLuc

Reference: source

Assay window 3.45

\(Z'\) 0.76

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

132 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

36 nM

raw data

Comment: CLK4-NLuc

Reference: source

Assay window 3.49

\(Z'\) 0.95

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

349 nM

raw data

Tracer displacement

i

Compound used for displacement:

Dasatinib

\(\mathrm{EC}_{50}\):

4 nM

raw data

Comment: EPHB1-NLuc

Reference: source

Assay window 3.79

\(Z'\) 0.88

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

258 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

420 nM

raw data

Comment: FES-NLuc

Reference: source

Assay window 2.44

\(Z'\) 0.79

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

83 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

\(\mathrm{EC}_{50}\):

30 nM

raw data

Comment: FLT1-NLuc

Reference: source

Assay window 3.25

\(Z'\) 0.73

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

21 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

\(\mathrm{EC}_{50}\):

4 nM

raw data

Comment: FLT3(K663Q)-NLuc

Reference: source

Assay window 3.15

\(Z'\) 0.76

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

181 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

\(\mathrm{EC}_{50}\):

182 nM

raw data

Comment: IGF1R-NLuc

Reference: source

Assay window 3.27

\(Z'\) 0.81

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

150 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

\(\mathrm{EC}_{50}\):

394 nM

raw data

Comment: https://www.promega.de/products/cell-signaling/kinase-target-engagement/insr-nanoluc-fusion-vector/?catNum=NV3271

Reference: source

Assay window 2.01

\(Z'\) 0.82

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

127 nM

raw data

Tracer displacement

i

Compound used for displacement:

Pacritinib

\(\mathrm{EC}_{50}\):

2 µM

raw data

Comment: NLuc-IRAK1

Reference: source

Assay window 3.97

\(Z'\) 0.88

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

138 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

87 nM

raw data

Comment: KIT(D816V)-NLuc

Reference: source

Assay window 3.11

\(Z'\) 0.68

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

5 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

2 nM

raw data

Comment: LRRK2-NLuc

Assay window 2.42

\(Z'\) 0.76

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

4 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

1 nM

raw data

Comment: LRRK2(G2019S)-NLuc

Reference: source

Assay window 3.52

\(Z'\) 0.74

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

20 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

2 nM

raw data

Comment: LRRK2(I2020T)-NLuc

Reference: source

Assay window 2.65

\(Z'\) 0.65

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

3 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

2 nM

raw data

Comment: LRRK2(R1441C)-NLuc

Reference: source

Assay window 5.99

\(Z'\) 0.73

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

244 nM

raw data

Tracer displacement

i

Compound used for displacement:

TAE-684

\(\mathrm{EC}_{50}\):

106 nM

raw data

Comment: LTK-NLuc

Reference: source

Assay window 3.19

\(Z'\) 0.92

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

188 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

\(\mathrm{EC}_{50}\):

975 nM

raw data

Comment: NLuc-MAP3K12

Reference: source

Assay window 2.11

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

43 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

\(\mathrm{EC}_{50}\):

1 µM

raw data

Comment: NLuc-MAP3K13

Reference: source

Assay window 4.94

\(Z'\) 0.79

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

256 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

7 nM

raw data

Comment: NLuc-MARK2

Reference: source

Assay window 2.93

\(Z'\) 0.81

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

175 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

\(\mathrm{EC}_{50}\):

932 nM

raw data

Comment: NLuc-MARK3

Reference: source

Assay window 3.79

\(Z'\) 0.88

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

193 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

239 nM

raw data

Comment: NLuc-MELK

Reference: source

Assay window 3.36

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

577 nM

raw data

Tracer displacement

i

Compound used for displacement:

CEP-701

\(\mathrm{EC}_{50}\):

198 nM

raw data

Comment: NLuc-MELK(T460M)

Reference: source

Assay window 2.51

\(Z'\) 0.77

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

91 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

239 nM

raw data

Comment: MERTK-NLuc

Reference: source

Assay window 2.47

\(Z'\) 0.84

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

144 nM

raw data

Tracer displacement

i

Compound used for displacement:

Foretinib

\(\mathrm{EC}_{50}\):

6 nM

raw data

Comment: MERTK(A708S)

Reference: source

Assay window 4.05

\(Z'\) 0.9

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

259 nM

raw data

Tracer displacement

i

Compound used for displacement:

Crizotinib

\(\mathrm{EC}_{50}\):

339 nM

raw data

Comment: MST1R-NLuc

Reference: source

Assay window 0.77

\(Z'\) 0.77

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

81 nM

raw data

Tracer displacement

i

Compound used for displacement:

CC1

\(\mathrm{EC}_{50}\):

4 µM

raw data

Comment: NEK6-NLuc

Reference: source

Assay window 2.3

\(Z'\) 0.68

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

200 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

1 nM

raw data

Comment: PAK1(M248-H545)-NLuc fusion

Assay window 2.88

\(Z'\) 0.68

CP agreement yes

Tracer titration

\(K_\mathrm{D}\):

225 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{IC}_{50}\):

0 nM

raw data

Comment: PAK1(M248-H545)-NLuc fusion

Assay window 1.56

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

199 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

9 nM

raw data

Comment: NLuc-PAK2(D229-R524)

Assay window 2.02

\(Z'\) 0.84

CP agreement yes

Tracer titration

\(K_\mathrm{D}\):

131 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{IC}_{50}\):

4 nM

raw data

Comment: NLuc-PAK2(D229-R524)

Assay window 2.28

\(Z'\) 0.66

CP agreement yes

Tracer titration

\(K_\mathrm{D}\):

263 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{IC}_{50}\):

1 nM

raw data

Comment: PAK3(D263-R559)-NLuc

Assay window 2.34

\(Z'\) 0.79

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

174 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

\(\mathrm{EC}_{50}\):

79 nM

raw data

Comment: PIK3C3-NLuc

Reference: source

Assay window 4.29

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

148 nM

raw data

Tracer displacement

i

Compound used for displacement:

BI-2536

\(\mathrm{EC}_{50}\):

13 nM

raw data

Comment: NLuc-PLK2

Reference: source

Assay window 5.17

\(Z'\) 0.93

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

327 nM

raw data

Tracer displacement

i

Compound used for displacement:

BI-2536

\(\mathrm{EC}_{50}\):

26 nM

raw data

Comment: NLuc-PLK3

Reference: source

Assay window 3.02

\(Z'\) 0.84

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

209 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

157 nM

raw data

Comment: PRKG2-NLuc

Reference: source

Assay window 3.52

\(Z'\) 0.95

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

127 nM

raw data

Tracer displacement

i

Compound used for displacement:

KW-2449

\(\mathrm{EC}_{50}\):

323 nM

raw data

Comment: NLuc-RIPK1

Reference: source

Assay window 2.0

\(Z'\) 0.8

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

81 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

\(\mathrm{EC}_{50}\):

3 µM

raw data

Comment: SGK2-NLuc

Reference: source

Assay window 3.01

\(Z'\) 0.77

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

138 nM

raw data

Tracer displacement

i

Compound used for displacement:

KW-2449

\(\mathrm{EC}_{50}\):

546 nM

raw data

Comment: STK17B-NLuc

Reference: source

Assay window 3.31

\(Z'\) 0.86

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

256 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

\(\mathrm{EC}_{50}\):

2 µM

raw data

Comment: STK24-NLuc

Reference: source

Assay window 5.26

\(Z'\) 0.69

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

142 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

\(\mathrm{EC}_{50}\):

1 µM

raw data

Comment: STK26-NLuc

Reference: source

Assay window 2.34

\(Z'\) 0.9

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

121 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

338 nM

raw data

Comment: NLuc-STK32A

Reference: source

Assay window 5.61

\(Z'\) 0.94

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

251 nM

raw data

Tracer displacement

i

Compound used for displacement:

Staurosporine

\(\mathrm{EC}_{50}\):

46 nM

raw data

Comment: NLuc-STK32B

Reference: source

Assay window 2.14

\(Z'\) 0.72

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

74 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

\(\mathrm{EC}_{50}\):

335 nM

raw data

Comment: NLuc-TSSK1B

Reference: source

Assay window 3.54

\(Z'\) 0.85

CP agreement yes

Tracer titration

\(K_\mathrm{D}^{\,\mathrm{app}}\):

183 nM

raw data

Tracer displacement

i

Compound used for displacement:

PBI 7727

\(\mathrm{EC}_{50}\):

2 µM

raw data

Comment: TTK-NLuc

Reference: source