|T000013, nsp10/16 RNA Tracer
ACCCCCN7-meGpppFAM

T000013

nsp10/16 RNA Tracer

  • Description

    Sequence:

    N7-meGppp-ACCCCC-FAM

    Dye:

    Fluorescein
  • Properties

    Store at:

    -80 °C

    Absorption:

    493 nm

    Emission:

    517 nm
  • References

    Synthesis:

    Publication

    Contributing Lab:

    Arrowsmith Lab, University of Toronto

  • Experimental

    Assay window 5.0
    \(Z'\) 0.6
    CP agreement yes
    Protein titration

    \(K_\mathrm{D}\):

    86 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    unlabelled N7 meGpppG

    \(\mathrm{IC}_{50}\):

    19 µM
    displacement curve
    raw data

    Comment: nsp10-nsp16 complex

    Reference: PMID