|T000012, miRNA-145 Tracer
GCUACGCGAUGCUAUAUAUACGCGCGAUGCGCAUAUUACGCGCGUA5'FITC3'5'

T000012

miRNA-145 Tracer

  • Description

    Sequence:

    GUCCAGUUUUCCCAGGAAUCCCU-FITC

    Dye:

    Fluorescein
  • Properties

    Store at:

    -80 °C

    Absorption:

    495 nm

    Emission:

    519 nm
  • References

    Contributing Lab:

    Arrowsmith Lab, University of Toronto

  • Experimental

    Assay window 10.0
    \(Z'\) 0.7
    CP agreement yes
    Protein titration

    \(K_\mathrm{D}\):

    173 nM
    titration curve
    raw data
    Tracer displacement
    i

    Compound used for displacement:

    unlabelled miRNA 145

    \(\mathrm{IC}_{50}\):

    94 nM
    displacement curve
    raw data

    Reference: PMID